Coding

Part:BBa_K129003:Design

Designed by: Burak YILMAZ   Group: iGEM08_METU_Turkey   (2008-11-08)


LamB-maltose transporter membrane protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1286
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1287
    Illegal PstI site found at 1301
    Illegal NotI site found at 7
    Illegal NotI site found at 1294
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1287
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1287
    Illegal PstI site found at 1301
    Illegal AgeI site found at 98
    Illegal AgeI site found at 1163
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LamB tolerates insertions of long heterologous peptides at a permissive loop (between structural codons 153 and 154) exposed to the external medium without a loss of function ). Successful attempts to introduce polyhistidine tails to LamB have been reported


Source

LOCUS A31943 1263 bp DNA linear PAT 20-JUN-1996 DEFINITION E.coli lamB gene fragment. ACCESSION A31943 VERSION A31943.1 GI:1567229 KEYWORDS . SOURCE Escherichia coli 

ORGANISM  Escherichia coli

          Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;

          Enterobacteriaceae; Escherichia.

REFERENCE 1 (bases 1 to 1263) 

AUTHORS   Hofnung,M., Bouges-Bocquet,B. and Guesdon,J.L.


References